Kolamu wili

Kolamu wili

Hōʻike Pōkole:

Hoʻopiha ʻia ke kolamu wili me ka nui o ka membrane puluniu aniani, i hoʻokumu ʻia ma ke ʻano o ka unuhi ʻana o ke aniani aniani no ka ʻenehana hoʻoiho acidic wikiwiki. Hiki ke hoʻohana i nā kolamu hoʻomaʻemaʻe e hoʻomaʻemaʻe i hoʻokahi - a i ʻole DNA pālua ʻia a i ʻole RNA mai nā impurities i loaʻa nā paʻakai, nā mea hoʻoheheʻe, nā ʻākena, a i ʻole nā ​​protein. Hoʻopili ʻia ka waikawa nukelika i ka fiber aniani ma kahi paʻakai kiʻekiʻe, a hoʻoneʻe ʻia ka paʻakai a me nā haumia i ka hana hoʻomaʻemaʻe. Hoʻomaʻemaʻe ʻia ka DNA / RNA maʻemaʻe i ka membrane me ka wai a i ʻole ʻo buffer TE. Me nā ʻano o ka hana maʻalahi, ka helu hoʻihoʻi kiʻekiʻe a me ka hana paʻa, ua hoʻohana ʻia ka huahana e ka hapa nui o nā hale hana a me nā hui ma ka home a me nā ʻāina ʻē. Hoʻohana ka kolamu hoʻomaʻemaʻe waikawa nukelika i ka membrane gel silica e like me nā mea adsorption kikoʻī o ka nucleic acid, ʻoiai ʻaʻole ka adsorption o nā mea olaola ʻē aʻe, i hiki ke hōʻoia i ka hoʻōla kiʻekiʻe o DNARNA i ka laʻana, ʻoiai e hemo ana i nā haumia ʻē aʻe.


ʻIke kikoʻī

Nā huahana huahana

Nā huahana huahana

Kolamu micropurification DNA / RNA

Hoʻohuli me nā buffer QIAGEN a me Invitrogen

ʻO ka paʻa paʻa he 45 ~ 50 g

Loaʻa ka nui o ka ʻāpana mai 65bp a i 10kbp

Kūpono ke kinikini buffer no ka hoʻomākaukau liʻiliʻi ʻana o ka plasmid, hemo ʻana i ka gel a me ka hoʻomaʻemaʻe PCR

Hoʻohana huahana

Hoʻomaʻemaʻe wikiwiki o nā huahana hoʻonui PCR

Loaʻa hou i nā kaula DNA mai ka agarose gel

Ka huki DNA Plasmid

Ka unuhi ʻana o Genomic DNA

Hoʻomaʻemaʻe RNA

Hoʻokaʻawale ʻana i kahi DNA kikoʻī i ka hoʻowahāwahā ʻia

Nā hiʻohiʻona huahana

ʻO ka hana maʻalahi a me ka pono o ka unuhi ʻana kiʻekiʻe.

Kaha huki kiʻekiʻe.

ʻO ka genomic DNA / RNA i kāʻili ʻia he maikaʻi maikaʻi a me ka maʻemaʻe kiʻekiʻe.

ʻO ke kiʻi ʻoniʻoni silica gel kiʻekiʻe kiʻekiʻe, hana hoʻolaha adsorption DNA / RNA maikaʻi.

Koho 4.6.8.12 koho

Hana ʻia ka ʻōmole hōʻiliʻili o ka kolamu hoʻomaʻemaʻe waikawa nucleic o polypropylene papa olakino

Hana ʻia ke kolamu o ka kolamu hoʻomaʻemaʻe waikawa nucleic o ka polypropylene papa olakino me ka hei a i ʻole ke kikowaena ma lalo.

ʻO ka gasket o ka kolamu hoʻomaʻemaʻe waikawa nucleic kahi mea fibrous kūikawā, e pale i ka waikawa a me ke kumu a me ka hapa nui o nā mea hoʻoheheʻe kūlohelohe, a ʻaʻole ia e hoʻolaha i ka nui o nā moleolaolaola.

ʻO ke kiʻi ʻoniʻoni silica gel i hoʻomākaukau hoʻomākaukau ʻia a i ʻole ke kiʻi aniani puluniu o nā mea i lawe ʻia mai i hiki ke adsorb nukelika acidic moleola i ka nui loa.

AʻOLE. Wehewehe Nā kānana kānana Waihona apo Kānana Kāpena Volume Hāʻawe / Ctns
ʻO PC0001 Kolamu Milo / / / 2ml Nui 10000
Eke 8000
PC0008 Kolamu Milo 2 Polū Nā olonā aniani 0.8ml Nui 12000
Eke 10000
ʻO PC0009 Kolamu Milo 4 ulaʻula Nā olonā aniani 0.8ml Nui 12000
Eke 10000
PC0010 Kolamu Milo 1 / Kānana PTFE 0.8ml Nui 12000
Eke 10000
PC0019 96 papa hoʻoiho maikaʻi 6 Polū ʻO ka silica membrance × 96 * 1.0ml Eke 50
PC0027 Kolamu Milo 8 Poni ʻO ka silica membrance 0.8ml Nui 12000
Eke 10000
PC0028 Kolamu Milo 4 Transparent ʻO ka silica membrance 0.8ml Nui 12000
Eke 10000
PC0033 Kolamu Milo 6 ulaʻula ʻO ka silica membrance 0.8ml Nui 12000
Eke 10000
ʻO PC0054 Kolamu Milo 12 ʻUlaʻula ʻulaʻula ʻO ka silica membrance 0.8ml Nui 12000
Eke 10000
PC0091 Kīpuka-e / / NC x / Eke 5000

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